Hydrogen molecules act as selective antioxidants by neutralizing reactive oxygen species (ROS), such as hydroxyl radicals (•OH) and peroxynitrite anions (ONOO⁻), converting them into harmless water molecules through redox reactions.

Following the elimination of harmful free radicals, cellular viability and physiological homeostasis may be restored. Improved cellular function has been shown to support the recovery of various organ systems, with progressive benefits observable in clinical biochemical markers over time.

The physiologically acceptable oxygen concentration for human inhalation typically ranges between 20% and 40%. Prolonged exposure to oxygen levels exceeding 40% for more than 24 hours may pose health risks due to oxygen toxicity. Asclepius Meditec's hydrogen-oxygen nebulizer is engineered to deliver a gas mixture containing 33% oxygen—well within the medically recognized safety range for long-term inhalation.

Key Biological Properties of Hydrogen-Oxygen Therapy

Selective Antioxidant Action – Hydrogen selectively neutralizes harmful reactive oxygen species, particularly hydroxyl radicals (•OH), while preserving beneficial signaling molecules such as nitric oxide (NO•).

Enhanced Oxygen Utilization – Hydrogen (H₂) improves the cellular uptake and efficiency of oxygen (O₂), helping to relieve tissue hypoxia and support metabolic recovery.

Anti-Inflammatory Modulation – Hydrogen suppresses pro-inflammatory signaling pathways (e.g., NF-κB, NLRP3), reducing the expression of cytokines like IL-6 and TNF-α.

Systemic Multiorgan Benefits – Via systemic absorption, hydrogen-oxygen supports functional recovery across multiple organs, most notably the lungs, brain, heart, and kidneys.

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Major Organ System Disorders

Respiratory System

- Chronic Obstructive Pulmonary Disease (COPD)
- Air Pollution-Induced Pulmonary Injury (e.g. PM2.5 exposure)
- Pneumonia
- Asthma
- Ventilation-Induced Lung Injury

Oncology

- Hepatocellular Carcinoma
- Lung Cancer
- Colorectal Cancer
- Gastric Cancer
- Glioblastoma
- Pancreatic Cancer
- Breast Cancer

Neurological Disorders

- Ischemic Stroke
- Spinal Cord Injury
- Neonatal Hypoxic-Ischemic Encephalopathy
- Parkinson's Disease
- Alzheimer's Disease
- Traumatic Brain Injury (TBI)
- Subarachnoid Hemorrhage
- Cognitive Impairment

Musculoskeletal System

- Osteoarthritis
- Rheumatoid Arthritis
- Muscle Injury
- Bone Fracture Repair

Cardiovascular System

- Myocardial Ischemia-Reperfusion Injury
- Heart Failure
- Hypertension
- Cardiac Arrest / Cardiopulmonary Resuscitation
- Vascular Dementia
- Atherosclerosis

Renal and Reproductive

- Endometriosis
- Kidney Stones
- Chronic Kidney Disease (CKD)

Infectious and Immune

- Sepsis
- Systemic Inflammatory Response Syndrome (SIRS)
- Autoimmune Diseases (general category)
- Graft-Versus-Host Disease (GVHD)

Integrated Biomedical Research Divisions

Literature

Research Papers on Hydrogen-Oxygen Therapy

Hydrogen gas ameliorates acute alcoholic liver injury via anti-inflammatory and antioxidant effects and regulation of intestinal microbiota

Hydrogen gas alleviates lipopolysaccharide-induced acute lung injury and inflammatory response in mice

Molecular Hydrogen Mediates Neurorestorative Effects After Stroke in Diabetic Rats: the TLR4/NF-κB Inflammatory Pathway

Combating Oxidative Stress and Inflammation in COVID-19 by Molecular Hydrogen Therapy: Mechanisms and Perspectives

Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation. Stem Cell Research & Therapy

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Research Projects

Asclepius has conducted 300+ basic researches, 32 clinical trials (16 single/16 multi-center), published 102 papers, and secured 459 patents with China's top hospitals.

As of December 1, 2024

Single Center Clinical Trials

Multi Center Clinical Trials

Natural Clinical Program

Project Name	Collaborative Hospital	Case Number	Status
Alleviate Airway Stenosis and Dyspnea and Reduce Postoperative Airway Granulation	Institute of Respiratory Medicine, The First A liated Hospital of Guangzhou Medical University	35	Paper published
Research on the Protection of Hydrogen-Oxygen Mixed Gas Against Air Pollution (Hydrogen-Oxygen Mixed Gas Protection Against PM2.5 Smog)	Second Hospital of Hebei Medical University	100	Paper published
Postoperative Cognitive Dysfunction (POCD) in Elderly Patients	Tianjin Medical University General Hospital	40	Paper published
Postherpetic Neuralgia	Tianjin Medical University General Hospital	60	Completed
Non-Alcoholic Fatty Liver Disease (NAFLD)	Taishan Medical College	80	Completed
Diabetes	School of Public Health, Shanghai University of Traditional Chinese Medicine	120	Completed
Dysbiosis	Institute of Respiratory Medicine, The First A liated Hospital of Guangzhou MedicalUniversity	80	In progress
Insomnia in Malignant Cancer Patients	Tumor Institute, Fourth Military Medical University	50	In progress
Pneumonia	Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University	30	In progress
Allergic Rhinitis	Shanghai Tongji Hospital	40	In progress
Real World Study on Advanced Cancer	Cancer Hospital A liated to Fudan University, Jinan University	82	Paper published
Adjuvant Therapy for Sudden Sensorineural Hearing Loss (SSHL)	Special Medical Center of Strategic Resources Force	60	In progress
Obstructive Sleep Apnea Syndrome (OSAS)	Hebei University of Traditional Chinese Medicine	40	Ethics reviewed
Acute Ischemic Stroke	Loma Linda University Medical Center	40	In progress
Clinical Research Plan on Investigating the Therapeutic Impact of Early Hydrogen-Oxygen Mixed Gas Inhalation Therapy in Aneurysmal Subarachnoid Hemorrhage Patients	Beijing Tiantan Hospital A iated to Capital Medical University	206	In progress
Exploration of the Impactand Mechanism of Hydrogen-Oxygen Mixed Gas in Modulating the NF-κB/NLRP3 Pathway a san Intervention in Silicosis	Shandong First Medical University	50	In progress

Project Name	Collaboration Units	Sample Size	Status
Study on Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Allergic Rhinitis	Department of Otolaryngology, Beijing Military Region General Hospital of the Chinese People's Liberation Army Department of Otolaryngology, Tianjin Medical University General Hospital	152	Completed
Study on Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Acute Exacerbation COPD	The First A iated Hospital of Guangzhou Medical University First Hospital of Hebei Medical University Shanghai Pulmonary Hospital Shanghai Fifth People‘s Hospital Tianjin Medical University General Hospital Zhongshan Hospital A iated to Fudan University Shanghai Tenth People‘s Hospital First A iated Hospital of Zhengzhou University The Second Hospital of Shanxi Medical University Shanghai Oriental Hospital	108	Completed
Study on Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Severe Stable COPD	West China Hospital of Sichuan University The First A iated Hospital of Guangzhou Medical University The Second A iated Hospital of Guangzhou Medical University Second Hospital of Hebei Medical University Tianjin Medical University General Hospital Zhongshan Hospital A iated to Fudan University Shanghai Pulmonary Hospital	152	End of Enrollment
Study on the Effect of Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Severe Asthma	The First A iated Hospital of Guangzhou Medical University The Second A iated Hospital of Guangzhou Medical University Second Hospital of Hebei Medical University Tianjin Medical University General Hospital Zhongshan Hospital A iated to Fudan University	150	Patients screening and enrolling
Study on the Effect of Hydrogen-Oxygen Mixed Gasas Adjuvant Therapy for Bronchiectasis	The First A liated Hospital of Guangzhou Medical University West China Hospital, Sichuan University The Second A liated Hospital of Guangzhou Medical University Zhongshan Hospital, Fudan University Shanghai Pulmonary Hospital	120	Patients screening and enrolling
Study on the E ect of Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Non-Small Cell Lung Cancer (NSCLC)	The First Hospital of Hebei Medical University The Third Hospital of Hebei Medical Hospital The First A liated Hospital of Guangzhou Medical University Tianjin Medical University Cancer	168	Ethics reviewing
Study on Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for COVID-19(Retrospective)	Leishenshan Hospital C6(Guangdong Provincial Hospital of Traditional Chinese Medicine) Guangzhou Eighth People‘s Hospital Wuhan Hankou Hospital Wuxue First People‘s Hospital Wuhan Hanyang Hospital(Weifang Weien Hospital) Harbin Sixth Hospital Suihua Cancer Hospital Jingzhou Central Hospital Shishou Traditional Chinese Medicine Hospital Wuhan Pulmonary Hospital Leishenshan Hospital C2(Shanghai Fifth People‘s Hospital) Shenzhen Third People‘s Hospital The First A iated Hospital of Nanchang University Hubei Provincial Hospital of Integrated Traditional Chinese and Western Medicine Qiqihar First Hospital Jingzhou First People‘s Hospital The First A iated Hospital of Guangzhou Medical University	259	Paper published
Study on Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for COVID-19 (Prospective)	The First A iated Hospital of Guangzhou Medical University The Eighth People’s Hospital of Guangzhou The Third People’s Hospital of Shenzhen The Ninth People’s Hospital of Dongguan Wuhan Hankou Hospital Leishenshan Ward C2 (Shanghai Fifth People’s Hospital) Leishenshan Ward C2 (Shanghai Sixth People’s Hospital) Leishenshan Ward C1 (Shanghai First People’s Hospital) Leishenshan Ward C6 (Shanghai Yangpu District Central Hospital) Wuhan Third Hospita l(Shanghai Ninth People’s Hospital) Wuhan Pulmonary Hospital	151	Completed
Clinical Trial Evaluating the Efficacy and Safety of Hydrogen- Oxygen Mixed Gas Inhalation in the Recovery Phase of Severe/ Critically COVID-19 patients	The First A liated Hospital of Guangzhou Medical University Wuhan Jinyintan Hospital Wuhan Hospital of Integrated Traditional Chinese and West ern Medicine (Wuhan First Hospital)	198	Ethics reviewed
Assessing the Short and Long-term Efficacy of Hydrogen-Oxygen Mixed Gas in Acute Exacerbations of Chronic Obstructive Pulmonary Disease: A Prospective Multicenter Real-world Study	The First A liated Hospital of Guangzhou Medical University Zhongshan Hospital A liated to Fudan University First Hospital of Hebei Medical University Xiangya Hospital Shanghai Fifth People's Hospital Shanghai Oriental Hospital 50 participating research units are expected	1021	In progress
Evaluation of the Effectiveness and Safety of Hydrogen-Oxygen Mixed Gas for Rehabilitation of Functional Impairment in Discharged Novel Coronavirus Pneumonia Patients	Zhongshan Hospital A liated to Fudan University Shanghai Fifth People's Hospital Shanghai Tenth People's Hospital Shanghai Oriental Hospital Shanghai Pudong Hospital Ruijin Hospital A liated to Shanghai Jiaotong University School of Medicine Huashan Hospital A liated to Fudan University	216	In preparation to start
Evaluation of the Effectiveness and Safety of Hydrogen-Oxygen Mixed Gas as Adjuvant Therapy for Novel Coronavirus Pneumonia (COVID-19)	Ruijin Hospital A liated to Shanghai Jiaotong University School of Medicine Zhongshan Hospital A liated to Fudan University Huashan Hospital A liated to Fudan University Ninth People's Hospital A liated to Shanghai Jiaotong University School of Medicine	188	Enrolling
Investigation on utilising Hydrogen-Oxygen Mixed Gas Inhalation as Intervention for Symptom Improvement in Children with Autism: A Multicenter, Non-randomized, Open-Parallel Controlled Clinical Trial	Shanghai Dongfang Hospital (Dongfang Hospital A liated to Tongji University) Binzhou People's Hospital of Shandong Province Binzhou Best Children's Hospital, Shandong Province Tai'an Central Hospital of Shandong Province Zibo Changguo Hospital, Shandong Province Rehabilitation Hospital of Taihe Hospital, Shiyan, Hubei Province Jinan Hospital of Shandong Province People's Hospital of Jinan City, Shandong Province The Second Maternal and Child Health Hospital of Jinan City, Shandong Province Dezhou Maternal and Child Health Hospital, Shandong Province Dongying Traditional Chinese Medicine Hospital of Shandong Province (Dongying Shengli Hospital) Maternal and Child Health Hospital of Zibo City, Shandong Province Xintai City People's Hospital of Shandong Province Maternal and Child Health Hospital of Dongchangfu District, Liaocheng, Shandong Province Maternal and Child Health Hospital of Tai'an City, Shandong Province School of Arti cial Intelligence, Beijing University of Posts and Telecommunications School of Computer Science and Architecture, Shandong Jianzhu University	292	Enrolling
Prospective, Multicenter, Real-World Investigation of hydrogen-oxygen mixed gas as short- and long-term adjunctive therapy or acute exacerbations of COPD	The First A iated Hospital of Guangzhou Medical University Zhongshan Hospital A liated to Fudan University The First Hospital of Hebei Medical University Xiangya Hospital, Central South University Shanghai East Hospital, Tong Ji University Shanghai Fifth People‘s Hospital Tianjin Medical University General Hospital Shanghai Tenth People's Hospital Second Hospital of Shanxi Medical University	214	Enrolling
Post-marketing study of AECOPD	The First A ated Hospital of Guangzhou Medical University Zhongshan Hospital A iated to Fudan University First Hospital of Hebei Medical University Tianjin Medical University General Hospital Hospital of Shanxi Medical University	216	In progress
A prospective, multicenter, randomized, parallel-controlled, double-blind clinical trial to evaluate the efficacy and safety of hydrogen-oxygen therapy in improving cancer-related fatigue (CRF)	Beijing Cancer Hospital Shandong Cancer Hospital Henan Cancer Hospital	158	Ethics reviewed
Sleep	Huashan Hospital Affiliated to Fudan University, East China Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai Tenth People's Hospital, The First Hospital of Hebei Medical University		Study Protocal Approving
Pulmonary Nodules	Zhongshan Hospital Affiliated to Fudan University Shanghai Oriental Hospital Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai Fifth People's Hospital Shanghai Tenth People's Hospital The First Affiliated Hospital of Guangzhou Medical University		Study Protocal Approving

Time (Year)	Collaboration Units	Project Name	Project No.
2015	Second Military Medical University of PLA	The protective effect and mechanism of water electrolysis-derived hydrogen on spinal cord injury in mice	81501052
2016	Second Military Medical University of PLA	Antioxidant effect of hydrogen on the immune response from asthmatic airway mucosa and its mechanism	81670017
	Taishan Medical University	Molecular mechanisms by which hydrogen regulates macrophage polarization and cholesterol metabolism	81670422
	Second Military Medical University of PLA	Study of hydrogen treatment on the prevention of endometriosis by inhibiting the ROS/PI3K/Akt pathway	81577355
	Guangzhou Medical University	The role and the possible mechanism of inhaled hydrogen gas in the prevention and treatment of COPD through regulating BMPR2expressio	81670041
2017	Taishan Medical University	Protective mechanism of molecule hydrogen on metabolic syndrome induced dysfunctional HDL	81770855
2018	Guangzhou Medical University	Effect of oxidative stress induced intestinal dysbacteriosis on chronic obstructive pulmonary dystrophy and its prevention and treatment	81873408
2019	Tongji University	A new mechanism of Hydrogen-Oxygen Mixed Gas intervention in pressure ulcers: Nrf2/ARE signaling pathway regulates premature aging and functional homeostasis of skin broblasts	81903191

Cooperating Hospitals

Anhui University of Chinese Medicine

Beijing Anzhen Hospital, Capital Medical University

Beijing Chao-Yang Hospital, Capital Medical University

Tianjin Medical University General Hospital

Shanghai Sixth People's Hospital

Guangzhou Fuda Cancer Hospital

Guangdong Provincial People's Hospital

Shanghai Armed Police Corps Hospital

Anhui University of Chinese Medicine

Beijing Anzhen Hospital, Capital Medical University

Beijing Chao-Yang Hospital, Capital Medical University

Tianjin Medical University General Hospital

Shanghai Sixth People's Hospital

Guangzhou Fuda Cancer Hospital

Guangdong Provincial People's Hospital

Shanghai Armed Police Corps Hospital

Team of Academicians and Professors

Professor Zhong Nan Shan

Fellow of the Royal College of Physicians of Edinburgh
The leading advisor in managing COVID-19 and SARS
Lifetime Honorary Fellow of theEuropean Respiratory Society

Professor Wang Zhen Yi

Professor Emeritus of Medicine and Pathophysiology at SJTU.
Discovered the cure for acute promyelocytic leukemia
Foreign associate of Acad mie des sciences (France)

Professor Wu Meng Chao

Known as the "Father of Chinese Hepatobiliary Surgery"
Invented the operation of intermittent interruption of the Porta Hepatis at room temperature
Awarded the State Preeminent Science and Technology Award

Dr Grace Russell

Certified Naturopathic Practitioner, accredited by The International Practitioners of Holistic Medicine
Research and education advisor for the molecular hydrogen institute (MHI)